Drosphilia Protocol

Drosphilia Protocol Observations and Procedure: My double mutant locomote atomic number 18 white eyed and have underlying travel in comparison to the groundless type flies. I plan on determining how these features atomic number 18 transmitted by means of genes is as follows: mensuration One: agnate extension I obtained a number of ten double mutant perfect(a) females from my carry ampul by clearing out exclusively the adults and capturing on the whole the new hatched females. I put all trinity new females in a separate vial with sise frenzied type males. All, except one, female died within the week with almost of my wild type males flies. I was still gibe to take hold the rest alive and larvae had been produced about a week later. P propagation:                                    Females                           Males Phenotype                            pureness eye                            brutal instance                                     undeveloped Wings                   Genotype                            secret                           Unknown Step both: F1 Generation Ten days later my original insure between the double mutant virgin female flies and the male wild type flies, my F1 genesis began to emerge. My totals and observations argon as follows: F1 Generation:                                    Females                           Males Phenotype                            sick emblem                           White Eyes     !                                                                     eagle-eyed Wings Possible Genotype                  Xw+vg X                           Xwvg Y chalk up # Collected Ratios of Phenotypes 1:1 Xw „³ White eye, X-linked recessive allele allelomorph Xvg „³ fundamental wings, Autosomal recessive Allele Xwvg „³ White eyeball, vestigial wings, X-linked recessive, Autosomal recessive alleles Xw+ „³ Wild type allele Y „³ No alleles for X-linked traits be carried on the Y chromosome Punnett Squares         Xwvg                  Xwvg X++         Xwvg X++         Xwvg X++ Y         Xwvg         Y         Xwvg Y found on my observations of the F1 Generation, the phenotypes and genotypes of the Parental Generation are likely to be: Parental Generation         :                                                                        Females                           Males Phenotype                           White eyes                           Wild Type                                    Vestigial Wings                   Genotype                           Xwvg Xwvg                           X++ Y The white eyes are found and in the males in the F1 coevals, enchantment the vestigial wings did not show up in the males of the F1 coevals. All of my females in the F1 generation ended up organism wild type. I conceive that the w! hite eyes are a X-linked recessive trait while the vestigial wings are an autosomal recessive trait. My next cross get out be between the F1 females and the F1 males. I can observe the traits in the F2 geneartion to prove or disprove my hypothesis.

Step 3: F2 Generation: As of now, I have done a F1 x F1 cross with no F2 emergence, so the following information I anticipate to go past regarding my F2 generation. I expect all my F1 wild type females are carriers for the X-linked recessive trait. Therefore crossing them with my F1 males should dedicate the following results: F2 Generation                                    Female                                             Male Phenotype                           Wild Type                                    Wild Type                   Genotype                           Xw+vg Xwvg                                    Xw+vg Y Total                                    50% of females                           50% of males Punnett Squares                  Xw+vg Xwvg Xw+vg         Xw+vg Xw+vg Y                  Xw+vg Y          Xw+vg Y                   Plan for abutting Step: B ased on the results of my F1 generation and F2 genera! tion, I would be done with my crosses. I would want to obtain a wide-ranging number of F2 generation specimens to increase my accuracy when I do my Chi square off test. I will be able to to determine whether or not the traits of my flies are well-nigh linked on the X chromosome. If they are closely linked, I will be able to determine a stand for based on the frequency of recombinants. My final report would let in all the data and calculations in this report.                            If you want to get a abounding essay, order it on our website: OrderEssay.net

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